Analysis of T cell receptor Vβ gene usage in primary mixed lymphocyte reactions : evidence for directive usage by different antigen-presenting cells and Mls-like determinants on T cell blasts
✍ Scribed by Eva-lotta Larsson-sciard; Anna-lena Spetz-hagberg; Armanda Casrouge; Philippe Kourilsky
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 680 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Analysis of T cell receptor Vp gene usage in primary mixed lymphocyte reactions : evidence for directive usage by different antigen-presenting cells and Mls-like determinants on T cell blasts
The usage of four different Tcell receptor (TcR) Vp gene families within normal, non-primed Tcell populations in response to various types of antigen-presenting cells (APC) in primary mixed lymphocyte reaction has been studied. We demonstrate that distinct patterns of Vp gene usage are obtained within a givenT cell population in response to different types of APC with the same allo-H-2. When responder Tcells are stimulated with one type of allogeneic APC, from various H-2-disparate mice, the same Vp gene preference is observed. Furthermore, when H-2-and Mls-mismatched APC gene used as stimulators, the Mls-associated Vg6 and Vg8.1 gene families are highly elevated in response to both B and Tcell blasts from certain Mls-positive strains.The results demonstrate that different types of allogeneic APC have the capacity to generate biases inTcR Vg gene usage and imply that functional MIS-like determinants are presented byT cell blasts. The findings are discussed with respect to TcR-major histocompatibility complex interactions in allostimulation.