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Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

โœ Scribed by Hasan, Nazarul (author);Humphrey, David (author);Riggs, Krista (author);Hu, Chuan (author)

Book ID
118735561
Publisher
Journal of Visualized Experiments
Year
2012
Tongue
English
Weight
296 KB
Volume
68
Category
Article
ISSN
1940-087X

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We have studied the kinetics of low pH-induced fusion between influenza virus A/PR 8/34 and human erythrocyte membranes in suspension by using an assay based on fluorescence dequenching (FDQ) of the lipophilic dye octadecylrhodamine B chloride (R 18). As shown previously (Clague et al. 1991) the ons