✦ LIBER ✦

Analysis of Single Cells with Capillary Electrophoresis Electrospray Ionization Fourier Transform Ion Cycloton Resonance Mass Spectrometry

✍ Scribed by Steven A. Hofstadler; Joanne C. Severs; Richard D. Smith; Franklin D. Swanek; Andrew G. Ewing

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 378 KB
Volume: 10
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19960610)10:8<919::aid-rcm597>3.0.co;2-8

No coin nor oath required. For personal study only.

✦ Synopsis

Recent results with on-line capillary electrophoresis (CE) electrospray ionization (ESD

Fourier transform ion cyclotron resonance (FTICR) mass spectrometry suggest that CE/ESI-FTICR can provide a powerful technique for micro-sample analyses owing to the inherent sensitivity of the technique and the enhanced information content derived from high-performance mass measurements. Using micro-sampling methods and ion accumulation techniques based on quadrupolar excitation, we demonstrate that adequate sensitivity exists to characterize the hemoglobin from a single human erythrocyte ( 4 5 0 -01).

In these studies mass spectra with average mass resolution in excess of 45 OOO (FWHM) were obtained for both the a-and f3 chain of hemoglobin following in-column lysing of a single erythrocyte.

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