✦ LIBER ✦
Analysis of reducing end-groups produced by enzymatic scission of glycosidic linkages in O-methylcellulose
✍ Scribed by Arifumi Nishimura; Yuji Matsumoto; Gyosuke Meshitsuka; Atsushi Ishizu
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 345 KB
- Volume
- 267
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
O-Methylcellulose (ds 1.8) was subjected to hydrolysis by cellulase and the portion that was enzymatically cleaved was analyzed. End-group analysis of the enzymatic hydrolyzate gave direct evidence for scission at the glycosidic linkages of 6-O-methyl-, 2-O-methyl-, and also unsubstituted glucose residues. The rates of cleavage of glycosidic linkages was: unsubstituted glucose residues > 6-O-methylglucose residues > 2-O-methylglucose residues.