Analysis of proteins by high-performance liquid chromatography with circular dichroism spectrophotometric detection
β Scribed by Yasuyuki Kurosu; Toru Sasaki; Takashi Takakuwa; Nobuyuki Sakayanagi; Kiyokatsu Hibi; Masaaki Senda
- Book ID
- 108337126
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 484 KB
- Volume
- 515
- Category
- Article
- ISSN
- 1873-3778
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The application of a circular dichroism (c.d.) detection system in HPLC using a chiral stationary phase is presented. The simultaneous measurement of the absorbance and c.d. signal allows the evaluation of the anisotropy factor (g = delta epsilon/epsilon) and thus the determination of the enantiomer
A new procedure using high-performance liquid chromatography for the rapid separation of cellulase proteins is described. The cellulase components of Trichoderma reesei are fractionated on a DEAE anion-exchange column using a phosphate buffer at pH 6.2. Activities of the individual components obtain