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Analysis of protein/polypeptide interactions in human plasma using nondenaturing micro-2-DE followed by 3-D SDS-PAGE and MS

โœ Scribed by Takashi Manabe; Ya Jin


Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
482 KB
Volume
28
Category
Article
ISSN
0173-0835

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Alkaline extraction of human plasma prot
โœ Ya Jin; Takashi Manabe ๐Ÿ“‚ Article ๐Ÿ“… 2007 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 385 KB

## Abstract Previously, we have reported a highโ€efficiency method of protein extraction from CBBโ€stained polyacrylamide gels for molecular mass measurement with MALDIโ€TOF MS [1]. In the present work, the alkaline extraction method was applied to CBBโ€stained 2โ€DE gels on which human plasma proteins