## Abstract Low repeatability of migration time, peak area, and linearity (p__I vs__. mobilization time) is a problem often encountered in capillary IEF (cIEF) and is mainly caused by protein precipitation and protein–wall interactions. In order to study the influence of these phenomena, the effect
✦ LIBER ✦
Analysis of protein fractions by micropreparative capillary isoelectric focusing and matrix-assisted laser desorption time-of-flight mass spectrometry
✍ Scribed by F. Foret; O. Müller; J. Thorne; W. Götzinger; B.L. Karger
- Book ID
- 107785735
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 597 KB
- Volume
- 716
- Category
- Article
- ISSN
- 1873-3778
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On-line capillary isoelectric focusing CIEF ᎐electrospray ionization Ž . time-of-flight mass spectrometry ESI᎐TOFMS as a two-dimensional separation Ž system is employed for high-resolution analysis of model proteins myoglobin and . -lactoglobulin and human hemoglobin variants C, S, F, and A. The fo