Analysis of progress curves for enzyme-catalyzed reactions: application to unstable enzymes, coupled reactions and transient-state kinetics
β Scribed by Ronald G. Duggleby
- Book ID
- 113269533
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 592 KB
- Volume
- 1205
- Category
- Article
- ISSN
- 0167-4838
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π SIMILAR VOLUMES
A special mixing device for initiating enzyme-catalyzed reactions is used to rapidly achieve an unperturbed quasi-steady state. An on-line computer is employed to sample the initial conditions, the mixing time, and concentrations that change as a function of time during this quasi-steady state phase
The steady-state velocity equation is derived for the general reaction scheme containing n kinds of enzyme species connected by a network of reversible reaction steps. The general equation is represented by the net rate constants as well as the true rate constants of the individual reaction steps. U