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Analysis of Polymerase Chain Reaction Products by On-Line Liquid Chromatography−Mass Spectrometry for Genotyping of Polymorphic Short Tandem Repeat Loci

✍ Scribed by Oberacher, Herbert; Parson, Walther; Mühlmann, Roswitha; Huber, Christian G.

Book ID
118141601
Publisher
American Chemical Society
Year
2001
Tongue
English
Weight
150 KB
Volume
73
Category
Article
ISSN
0003-2700

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An on-line method has been developed for the derivatization and coupled liquid chromatography (LC)/electrospray ionization (ESI) MS analysis of peptides at the femtomol level. Peptides are reacted with N-succinimidyl-2(3-pyridyl)acetate (SPA) in buffered aqueous medium at pH 7 following loading on a