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Analysis of picogram quantities of indole-3-acetic acid by high performance liquid chromatography-fluorescence procedures

โœ Scribed by Alan Crozier; Karen Loferski; Joe B. Zaerr; Roy O. Morris

Book ID
104751823
Publisher
Springer-Verlag
Year
1980
Tongue
English
Weight
472 KB
Volume
150
Category
Article
ISSN
0032-0935

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โœฆ Synopsis

The use of spectrofluorimeter coupled to a reverse phase high performance liquid chromatography column permits selective detection of indole-3-acetic acid at the low picogram level. The value of the technique is demonstrated by the analysis of endogenous IAA in elongating shoots, xylem sap and callus of Douglas-fir. The data are also used to illustrate a procedure whereby the accuracy of chromatographic analyses can be verified within definable probability limits.

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Simultaneous analysis of indole-3-acetic
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โœ David Blakesley; John Frederick Hall; Geoffrey Donald Weston; Malcolm Charles El ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 697 KB

The use of high-performance liquid chromatography in the reversed-phase mode coupled to a spectrophotofluorimetric detector allowed the selective detection of acidic indoles possessing a C-3 methylene side chain, following the reaction of a purified plant extract with acetic anhydride. Of these acid