Analysis of physiological α-synuclein by non-denaturing gel electrophoresis
✍ Scribed by Liu, Feng; Li, Linglin; Ryder, John; Hole, Justin; Racke, Margaret; Merchant, Kalpana; DeMattos, Ronald
- Book ID
- 122602156
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 52 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1552-5260
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## Abstract After separation by microscale non‐denaturing two‐dimensional gel electrophoresis (2DE) and transferring to a blotting membrane, major proteins are detected by a staining of direct blue 71 in a neutral solution. The carboxylesterase on the membrane hydrolyzes phosphatidylcholine after t