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Analysis of Partially Methyl-Esterified Galacturonic Acid Oligomers by Capillary Electrophoresis

✍ Scribed by Martin A.K. Williams; Gaëlle M.C. Buffet; Tim J. Foster

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
74 KB
Volume
301
Category
Article
ISSN
0003-2697

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✦ Synopsis

Recent progress in the determination of the intramolecular distribution of methyl-esterified residues in pectic substrates has been made using a fragmentation approach in which endopolygalacturonase is used to digest the polysaccharide and its subsequent (methyl-ester sequence-dependent) digest pattern is determined. This has been facilitated by the separation of partially methyl-esterified enzyme digest fragments using high-performance anion-exchange chromatography at pH 5. Here we demonstrate that capillary electrophoresis can be used as an additional method for separation and quantification of such digest patterns. The technique offers improvements in speed and economy of materials and a straightforward quantification procedure.

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