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Analysis of nuclear RNA interference in human cells by subcellular fractionation and Argonaute loading

✍ Scribed by Gagnon, Keith T; Li, Liande; Janowski, Bethany A; Corey, David R


Book ID
127226210
Publisher
Nature Publishing Group
Year
2014
Tongue
English
Weight
874 KB
Volume
9
Category
Article
ISSN
1750-2799

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## Abstract Tissue factor (TF) is the membrane receptor of the serine protease coagulation factor VIIa (FVIIa). Formation of the TF/FVIIa complex initiates the coagulation cascade. We used short hairpin RNA (shRNA)‐mediated RNA interference to knock down TF expression in the human metastatic melano