Analysis of Noncovalent Complexes between Human Telomeric DNA and Polyamides Containing N-Methylpyrrole and N-Methylimidazole by Using Electrospray Ionization Mass Spectrometry
✍ Scribed by Jiang Zhou; Gu Yuan
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 135 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0947-6539
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✦ Synopsis
Abstract
Electrospray ionization mass spectrometry (ESI‐MS) was used to investigate noncovalent complexes formed between four novel polyamides containing N‐methylpyrrole (Py) and N‐methylimidazole (Im), and human telomeric DNA. Of the four polyamides investigated, PyPyPyγImImImβDp (3) had the highest binding affinity towards the duplex d(TTAGGGTTAGGG/CCCTAACCCTAA) (D1). Results of competition analysis showed that the polyamides had binding affinities with D1 in the order PyPyPyγImImImβDp (3)>PyPyPyPyγPyImImPyβDp (4)>PyPyPyβImImImβDp (2)≫ImImImβDp (1). MS/MS spectra confirmed that binding between D1 and the hairpin polyamides is more stable than that with the three‐ring polyamides. By contrast, in the case of single‐stranded d(TTAGGGTTAGGG)(D2), the binding order changes to ImImImβDp (1)>PyPyPyγImImImβDp (3)>PyPyPyβImImImβDp (2).