Analysis of myo-inositol phosphates by 2D 1H-n.m.r. spectroscopy

✍ Scribed by Charlotta Johansson; Johan Kördel; Torbjörn Drakenberg

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 494 KB
• Volume: 207
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(90)84047-x

✦ Synopsis

2D 'H-N.m.r. spectroscopy applied to phosphorylated myo-inositols can indicate the number and the sites of phosphorylation, and up to three isomers can be completely analyzed simultaneously. The resonance of the sole equatorial proton (H-2) is shifted furthest downfield (to -4.7 p.p.m.) when C-2 is phosphorylated; when C-5 is unphosphorylated, the H-5 resonance has the lowest chemical shift (N 3.3 p,p.m,). The number of sites of phosphorylation can be determined from the sum of the chemical shifts for the resonances in a given myo-inositol phosphate and, based on the data presented, an algorithm can be constructed that will identify any myo-inositol phosphate on the basis of the chemical shifts.