Analysis of mono- and disaccharides by high-performance liquid chromatography of the benzyloxime-perbenzoyl derivatives

✍ Scribed by Richard M. Thompson

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 693 KB
• Volume: 166
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)92263-5

✦ Synopsis

A group of biologically important mono-and disaccharides are separated by high-performance liquid chromatography of the benzyloxime-perbenzoyl derivatives on a normal-phase microparticulate column with a hexane-dioxane mixture as the eluting solvent. A single, quantifiable derivative of each sugar is formed easily. These derivatives are detected by UV absorption at either 230 or 254 m-n, with a sensitivity in the picomole range at the former wavelength. The sugars in the residues from the evaporation of small aliquots of biologic fluids (10-100~1) are derivatized without prior isolation and are determined quantitatively by the use of appropriate internal standards. The analyses could be performed routinely with a simple, inexpensive instrument.