✦ LIBER ✦

Analysis of Mammalian Cell Cytoplasm with Electrophoresis in Nanometer Inner Diameter Capillaries

✍ Scribed by Lori A. Woods; Paula R. Powell; Tracy L. Paxon; Andrew G. Ewing

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 141 KB
Volume: 17
Category: Article
ISSN: 1040-0397
DOI: 10.1002/elan.200403240

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✦ Synopsis

Capillary electrophoresis in 770 nanometer inner diameter capillaries coupled to electrochemical detection with an etched electrode matching an etched capillary (etched electrochemical detection) has been used with ultrasmall sampling to inject subcellular samples from intact single mammalian cells. Separations of cytoplasmic samples taken from rat pheochromocytoma cells have been achieved. As little as 8% of the total volume of a single cell has been sampled and analyzed. Dopamine has been identified and quantified in these PC12 cells using this technique. The average cytoplasmic level of dopamine in rat pheochromocytoma cells has been determined to be 240 AE 60 mM. The use of electrophoresis in 770 nanometer inner diameter capillaries with electrochemical detection to monitor cytoplasmic neurotransmitters at the single cell level can provide information about complex cellular functions such as neurotransmitter storage and synthesis.

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