Analysis of intracellular ribonucleotide pools in a drug-treated human cancer cell line using capillary zone electrophoresis
✍ Scribed by Shaofan Liu; James A. North; Byron K. Murray; Mingxian Huang; Milton L. Lee
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 386 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1040-7685
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Capillary zone electrophoresis was used to separate and identify intracellular ribonucleotides and nucleotide analogues from ribavirin‐ and guanine‐treated HeLa cells, a human cervical carcinoma cell line. Nine ribonucleotides in HeLa cell extracts were identified and quantitated. In cell extracts, the average ATP/ADP ratio was 6.5 and the average energy charge of the HeLa cells was 0.92. In HeLa cells treated with guanine, the GTP pool increased to 309% of control values. When cells were treated with ribavirin, the GTP pool was reduced to 33% of nondrug treated controls. Ribavirin triphosphate reached levels of 113.25 nmols/10^7^ cells, while ribavirin diphosphate reached levels of 4.96 nmols/10^7^ cells.