✍ Scribed by G. Holzer; T.F. Bourne; W. Bertsch
No coin nor oath required. For personal study only.
A newly developed Curie-point pyrolyzer has been used to analyze microbial fatty acid constituents. The pyrolysis was carried out on ferromagnetic wires, which were loaded into the gas chromatographic injector-pyrolyzer via a retractable Pyrex glass tube. Fatty acid methyl esters were obtained by in situ methylation of the free and glycerol bound fatty acids with trimethylanilinium hydroxide. The procedure was applied sucessfully to whole microorganisms. There was good agreement between the fatty acid methyl ester profiles from the whole-cell pyrolysis and from the lipid extracts of the corresponding organisms.
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Trimethylsilylated methyl esters of octadecadienoic, eicosadienoic, and octadecatrienoic acids, after stereospecific oxidation, were investigated by combined gas chromatography-chemical ionization mass spectrometry. Molecular weight could be determined by the ions at m/e(M -1)' and (M + 73)+ and the
Methyl-methine linkages of Novolac, a commercially available t-butylphenol acetylene condensed (TBPA) resin, have been identified by recognition of pyrolysis pathways using pyrolysis-gas chromatography/mass spectrometry (Py-GC/mS) in vulcanized rubber. The diagnostic mass spectrum of t-butylphenol w