Analysis of immunoglobulins expressing the Vx21E and Vx21D gene products. Evidence for multigenic control of the expression of VH−VL combinations
✍ Scribed by Daniele Primi; Christian Devaux; Michel Pierres; Eliane Barbier; Pierre-André Cazenave
- Book ID
- 102826409
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 733 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
The aim of this work was to study the genetic control of VH-VL combined expression. To this end immunoglobulins expressing the V kappa 21E and V kappa 21D gene products were isolated from the normal sera of several inbred strains of mice using a monoclonal antibody that selectively reacts with V kappa 21E and V kappa 21D subgroups. Analysis of the isoelectric focusing (IEF) pattern of the IgG heavy chains of these immunoglobulins obtained from H-2-congeneic mice revealed as expected that Igh-linked loci strongly influence VH-VL combined expression. The IEF pattern of V kappa 21D and V kappa 21E-associated heavy chains isolated from recombinant inbred mouse strains, however, revealed different phenotypes from either parental strains in strains in which both H-2 and Igh loci segregated from the same parent. These data, therefore, strongly suggest that the expression of VH-VL combinations is controlled in part by genetic loci which segregate independently from those linked to H-2 and Igh.