Analysis of hydrophilic metabolites in physiological fluids by HPLC-MS using a silica hydride-based stationary phase
✍ Scribed by Joseph J. Pesek; Maria T. Matyska; Joseph A. Loo; Steven M. Fischer; Theodore R. Sana
- Book ID
- 102926997
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 971 KB
- Volume
- 32
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
Aqueous normal‐phase chromatography was used for the analysis of metabolites in human saliva and urine samples. The column was packed with a silica hydride type separation material. Several gradients were tested with different mobile phase additives in order to produce retention for amino acids, small organic acids, and carbohydrates. Detection was done by TOF MS. In some cases the relative concentration levels of various metabolites in human saliva were compared for normal patients and patients with pancreatic cancer or pancreatitis. The reproducibility of retention of individual metabolites in these complex matrices was tested for several compounds.