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Analysis of heme biosynthesis in catalase and cytochrome deficient yeast mutants

✍ Scribed by Labbe-Bois, Rosine ;Rytka, Joanna ;Litwinska, Jadwiga ;Bilinski, Tomasz

Publisher: Springer
Year: 1977
Tongue: English
Weight: 611 KB
Volume: 156
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00283490

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✦ Synopsis

Mutants of Saccharomyces cerevisiae, described as catalase and cytochromes deficient (Pachecka et al., 1974), have been analyzed for heme biosynthesis ability. Some enzymatic activities involved in protoheme synthesis were measured in acellular extracts, whereas whole cells were analyzed for cytochrome spectra and for possible accumulation of porphyrin synthesis intermediates. A good correlation was found between these in vitro and in vivo studies. Results show that two mutants were impaired in 5-aminolevulinate synthesis, two mutants were devoid of uroporphyrinogen I synthetase activity and one mutant presented defects in coproporphyrinogen III oxidase activity.

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