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Analysis of green pit viper (Trimeresurus alborabris) venom protein by LC/MS-MS

✍ Scribed by Suphan Soogarun; Polkit Sangvanich; Montri Chowbumroongkait; Surasak Jiemsup; Viroj Wiwanikit; Paweena Pradniwat; Attakorn Palasuwan; Jaturawat Pawinwongchai; Supanthitra Chanprasert; Tusrin Moungkote

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
271 KB
Volume
22
Category
Article
ISSN
1095-6670

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✦ Synopsis

Abstract

Green pit viper venom has major effect on the hematological system having a thrombin‐like effect. Thus, this study is designed to analyze the composition of Trimeresurus albolabris venom by performing gel filtration and LC/MS‐MS. The purified protein was then digested by trypsin, and the tryptic fragments were analyzed by iontrap spectrophotometry. This study found four types of proteins, namely jerdonitin, stejaggregin‐A β chain‐1, stejnobin, and stejnihagin‐A, as the components of T. albolabris venom. All of these toxins played a greater or lesser role in clot formation or otherwise contributed to cross‐reactions in antivenom production. © 2008 Wiley Periodicals, Inc. J Biochem Mol Toxicol 22:225–229, 2008; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20232

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