Analysis of furosemide and its main metabolites in biological fluids by scanning according to the fluorescence of thin-layer chromatograms

Analysis of Bilirubins in Biological Flu

Analysis of Bilirubins in Biological Fluids by Extraction and Thin-Layer Chromatography of the Intact Tetrapyrroles: Application to Bile of Patients with Gilbert's Syndrome, Hemolysis, or Cholelithiasis

✍ Johan Fevery; Norbert Blanckaert; Pol Leroy; Roger Michiels; Karel P. M. Heirweg 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 725 KB

A method was developed to extract quantitatively the bilirubins from bile, urine, serum, stool, and preparations from liver with a chloroform-ethanol mixture at pH 1.8 in the presence of ascorbic acid and NaC1. Extracted pigment was submitted to thin-layer chromatography, and the separated bilirubin