Analysis of fluorescently labeled glycosphingolipid-derived oligosaccharides following ceramide glycanase digestion and anthranilic acid labeling

High-Performance Liquid Chromatography A

High-Performance Liquid Chromatography Analysis of Ganglioside Carbohydrates at the Picomole Level after Ceramide Glycanase Digestion and Fluorescent Labeling with 2-Aminobenzamide

✍ D.R. Wing; B. Garner; V. Hunnam; G. Reinkensmeier; U. Andersson; D.J. Harvey; R. 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 118 KB

The functional importance of glycolipids has emphasized the need for more sensitive methods of detection, characterization, and quantification than has often been possible using traditional thin-layer chromatographic techniques. We describe the use of ceramide glycanase and HPLC to identify and quan