Analysis of fluorescence lifetime curves: A new method for correcting raw fluorescence lifetime curves for the parallel contribution by scattered light
✍ Scribed by Kenneth Zierler; Ellen M. Rogus
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 509 KB
- Volume
- 95
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A method is described for estimating the fractional contribution of light scattered from the excitation lamp to the normalized raw fluorescence lifetime curve. The method depends on the ratio of the slope of the normalized light scatter spectrum to the slope of the normalized raw fluorescence spectrum in the vicinity of the intersection of the two spectra. The correction for scattered light is made prior to deconvolution, and hence, has the advantage of being independent of the method selected to calculate the true fluorescence lifetime spectrum. It is simple and does not require a computer. Tested against curves synthesized from known additions of scattered light to fluorescence spectra exhibiting mono-, bi-, or triexponential decay, it yielded small absolute errors.