Analysis of extracts from cedrela toona (meliaceae) by on-line and off-line supercritical fluid extraction-capillary gas chromatography

✍ Scribed by William K. Modey; Dulcie A. Mulholland; Hamdani Mahomed; Mark W. Raynor

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 695 KB
• Volume: 8
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1996)8:1<67::aid-mcs8>3.0.co;2-6

✦ Synopsis

A quantitative comparison between off-line supercritical fluid extraction (SFE) and a classical solvent extraction of a limonoid compound is discussed. A two-level, three factor factorial analysis was used to optimize the variables, pressure, temperature, and the length of extraction time. By a statistical analysis of variance on the preliminary data collected, pressure and temperature were the significant variables influencing the yields of the limonoid cedrelone from the ground wood of Cedrela toona (Meliaceae). The cedrelone in the extracts was analyzed by capillary gas chromatography with flame ionization and mass spectrometric detection. On-line combination of micro-SFE with gas chromatography (SFE-GC) was also performed on the wood and bark to show the applicability of the technique in the analysis of semi-volatile components in natural products. Results for on-line and off-line SFE-GC were comparable and indicated that the on-line studies could be used to predict results obtainable from large scale SFE.