Analysis of expression of heavy myosin chains during in vitro differentiation of satellite cells and myoblasts derived from rat skeletal muscles
β Scribed by O. V. Balan; N. S. Myuge; N. D. Ozernyuk
- Book ID
- 110213191
- Publisher
- Springer
- Year
- 2009
- Tongue
- English
- Weight
- 341 KB
- Volume
- 36
- Category
- Article
- ISSN
- 1062-3590
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The satellite cell is responsible for growth and repair of postnatal skeletal muscle. We investigated the expression of the myogenic regulatory gene (MRG) family in these cells in the stages from quiescence to fusion. Using polymerase chain reaction amplification of reverse-transcribed RNA (RT-PCR)
Myosin heavy chains (hcs) are the major determinant in the speed of contraction of skeletal muscle, and various isoforms are differentially expressed depending on the functional activity of the muscle. Using the rapid amplification of cDNA ends (3Π RACE) method, we have characterised the 3Π end of t