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Analysis of EPC-1 growth state–dependent expression, specificity, and conservation of related sequences

✍ Scribed by Robert J. Pignolo; Mitch O. Rotenberg; Vincent J. Cristofalo

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
921 KB
Volume
162
Category
Article
ISSN
0021-9541

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✦ Synopsis

The transcipt for EPC-I (early population doubling level (PDL) cDNA-1) is induced under conditions of growth arrest due to density-dependent contact inhibition and/or serum deprivation in early-passage but not in senescent WI-38 fibroblasts. We have characterized the EPC-I transcript with respect to its cell-cycle regulation, tissue specificity, and interspecies conservation of related genomic sequences. In low density, quiescent (serum-deprived), early-passage fibroblasts that are stimulated to proliferate with fresh serum, steady-state EPC-I transcript levels are steadily reduced until they reach a basal level approximately 24 h after stimulation. However, when early-passage fibroblasts are made quiescent by both serum deprivation and density-dependent contact inhibition and then stimulated with serum, steady-state EPC-I transcript levels remain relatively constant throughout a 36 h period following serum stimulation. Senescent WI-38 cells

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