Qualitative gas chromatography-mass spectrometry (GC-MS) analysis of purified extracts from 4-dgerminated seeds of "jacarandfi do cerrodo" (Dalbergia dolichopetala) detected gibberellin A 1 (GA1), epi-GA~, GA 3, GA4, GA 5, GA 8, GAz0 and GAzs. Quantitative analysis, using deuterated internal standards, showed that the germinated seed contained 29 ng GA1, 0.6 ng GA4, 0.08 ng GA 5 and 1.7 ng GA20.g ~ fresh weight. In metabolism experiments with germinated D. dolichopetala seeds, using 2H2, 3H-labelled GA1, GA4, GA 5 and GA2o , metabolic profiles were obtained by reversedphase high-performance liquid chromatography-radiocounting after which individual radiolabelled peaks were analysed by GC-MS and/or combined high-performance liquid chromatography-frit fast atom bombardmentmass spectrometry. The data obtained demonstrated the conversion of GA2o to GAz0 glucosyl ester, and also to GA~ which was further metabolised, presumably via GA 8, to an unidentified GA 8-O-glucoside with a mass spectrum different from those of the 2-0-and 13-0glucosides ofGA 8. Gibberellin A 1 was also conjugated to form GA1-3fl-O-glucoside and a conjugate identified tentatively as GA 1 -3fl-O-glucuronic methyl ester. Gibberellin A 4 was converted to GA 4-3fl-O-glucoside and GAl. Gibberellin A 5 was converted to GA 5 glucosyl ester, GA 3 and GA3-3fl-O-glucoside.