Analysis of DNA synthesis rate of cultured cells from flow cytometric data

✍ Scribed by Dr. A. Bertuzzi; A. Gandolfi; A. Germani; M. Spanò; G. Starace; R. Vitelli

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 819 KB
• Volume: 5
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/cyto.990050611

✦ Synopsis

The rate of DNA synthesis along S phase is estimated from flow cytometric histograms on the basis of a mathematical model of a cell population. In the absence of loss, the model expresses the population kinetics in terms of DNA synthesis rate, S-phase influx, and population size. A single histogram is sufficient to determine the DNA synthesis rate when the population is in balanced exponential growth. Two suitably chosen histograms are necessary if the S-phase influx is exponential in a time interval longer than the S-phase dura-tion. The analysis procedure was tested on published autoradiographic data and applied to three cultured cell lines (CM-S, 3LL, and M14 cells) that show various patterns of DNA distribution. In each case the cell-cycle fractions, the DNA synthesis rate, and the S-phase duration were obtained.