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Analysis of differential gene expression patterns in colon cancer and cancer stroma using microdissected tissues

✍ Scribed by Yuko Sugiyama; Buckminster Farrow; Carlos Murillo; Jing Li; Hiroaki Watanabe; Kazuo Sugiyama; B. Mark Evers

Book ID
119758235
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
141 KB
Volume
128
Category
Article
ISSN
0016-5085

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## Abstract To examine gene‐expression patterning in late‐stage breast cancer biopsies, we used a microdissection technique to separate tumor from the surrounding breast tissue or stroma. A DD‐PCR protocol was then used to amplify expressed products, which were resolved using PAGE and used as probe