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Analysis of dexamethasone, triamcinolone, and their metabolites in human urine by microcolumn liquid and capillary gas chromatography mass spectrometry

✍ Scribed by Rodchenkov, G. M. ;Uralets, V. P. ;Semenov, V. A. ;Leclercq, P. A.

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
475 KB
Volume
11
Category
Article
ISSN
0935-6304

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✦ Synopsis

Adult male volunteers were administered orally 10 mg of the respective drug. Urine samples were investigated by microcolumn liquid chromatography and capillary gas chromatography combined with on-line mass spectrometry. The former technique was found to be usable for the detection of these drugs. The latter method appeared to be superior in terms of sensitivity and for metabolism studies. The major excretion products of dexamethasone were identified as isomeric 6-hydroxy metabolites. Dexamethasone as such and its 20- hydroxy metabolite were found in minor quantities. Unlike dexamethasone, triamcinolone is excreted largely unchanged from the human body. Two metabolites were identified as 11- keto and 4,5-dihydrotriamcinolone. With the gas chromatographymethods developed, abuseof these drugscan bedetected up to 24 h after administration of a 5 mg single dose.

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