✦ LIBER ✦

Analysis of derivatized prostaglandins by capillary supercritical fluid chromatography with a phosphorus-selective detector

✍ Scribed by P. A. David; M. V. Novotny

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 116 KB
Volume: 10
Category: Article
ISSN: 1040-7685
DOI: 10.1002/(sici)1520-667x(1998)10:1<27::aid-mcs4>3.0.co;2-0

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✦ Synopsis

Model prostaglandins were reacted with dimethylthiophosphinic chloride to form suitable derivatives using dimethylaminopyridine as a catalyst for supercritical fluid chromatography with thermionic detection. The high sensitivity of this phosphorus-selective detector makes it feasible to detect biologically important prostaglandins and related metabolites in physiological fluids at nanogramper-milliliter concentrations.