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Analysis of conjugated bile acids by on-line supercritical fluid chromatography/thermospra mass spectrometry

✍ Scribed by Santo Scalia; David E. Games

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
421 KB
Volume
27
Category
Article
ISSN
1076-5174

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✦ Synopsis

A rapid method was developed for the analysis of polar conjugated bile acids by combined packed column supercritical fluid chromatography/mass spectrometry. Direct coupling of the column to the mass spectrometer was effected with a modified thermospray interface operated in the filament-on mode. Maximum sensitivity for the bile acid conjugates was achieved by recording the negative ions generated in the ionization process. The effects of vaporizer and source temperatures, repeller voltage and discharge conditions on the bile acid response were investigated. The mass spectra obtained for the common glycine and taurine conjugates yielded only the [ M -HIpseudo-molecular ions. In contrast with the taurine derivatives, the glycine forms produced a weak ion current and exhibited broad bands. The applicability of the technique is illustrated with a sample of human bile.

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