Analysis of clamps with time dependent voltages in the squid axon using a kinetic transport model

✍ Scribed by Michael E. Starzak

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 712 KB
• Volume: 57
• Category: Article
• ISSN: 0022-5193
• DOI: 10.1016/s0022-5193(76)80010-0

✦ Synopsis

A sequential kinetic, open system model for potassium currents in squid axon is used to develop analytical expressions for current fluxes during time dependent voltage clamp experiments. These analytical solutions suggest a technique for extracting rate constant data directly from the current voltage curves generated during such damps. The analytical solutions involve a simple decay scheme which provides a simple explanation for hysteresis effects observed with such clamps. In addition, the solutions predict a minimal time dependence for the hyperbolic voltage clamp, i.e., the observed current fluxes during a hyperbolic voltage clamp will tend to be independent of the rate of decay. The use of this clamp for the rapid separation of sodium and potassium currents is discussed.