Analysis of chromate-induced DNA-protein crosslinks with the comet assay
✍ Scribed by Oliver Merk; Kristin Reiser; Günter Speit
- Book ID
- 118626272
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 217 KB
- Volume
- 471
- Category
- Article
- ISSN
- 1383-5718
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The single cell gel electrophoresis, or comet assay, under alkaline conditions is a sensitive, simple and rapid method for the detection of DNA damage at the individual cell level. Its applicability as an indicator for the DNA crosslinking potency of a test substance was investigated in human white
## Background: The ability of the comet assay to quantify DNA strand breaks and alkali labile sites has been widely demonstrated. In this study, this assay was tested for its ability to identify DNA fragmentation occurring during apoptosis in comparison with standard DNA flow cytometry analysis. M
The alkaline comet assay is a sensitive test for the detection of a variety of DNA lesions. However, crosslinks are not readily detected under standard test conditions. Recently, modifications have been introduced measuring crosslinks by determining the reduction of induced DNA migration. We used th