Analysis of cell movement by simultaneous quantification of local membrane displacement and fluorescent intensities using Quimp2

Use of a membrane-localized green fluore

Use of a membrane-localized green fluorescent protein allows simultaneous identification of transfected cells and cell cycle analysis by flow cytometry

✍ Robert F. Kalejta; Thomas Shenk; Andrew J. Beavis 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 110 KB 👁 2 views

The simultaneous detection of the green fluorescent protein (GFP) and DNA content using propidium iodide (PI) by flow cytometry is made difficult because of the unique nature of these 2 fluorogenic reagents. For PI to enter cells efficiently and to stain DNA quantitatively, the cells must first be p