✦ LIBER ✦
Analysis of Carbon Metabolism in Escherichia coli Strains with an Inactive Phosphotransferase System by 13C Labeling and NMR Spectroscopy
✍ Scribed by S. Flores; G. Gosset; N. Flores; A.A. de Graaf; F. Bolı́var
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 255 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1096-7176
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✦ Synopsis
We have developed Escherichia coli strains that internalize glucose utilizing the GalP permease instead of the phosphoenolpyruvate:carbohydrate phosphotransferase system. It has been demonstrated that a strain with these modifications (PTS -Glc + ) can direct more carbon flux into the aromatic pathway than the wild-type parental strain (N.