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Analysis of calcium binding to α-lactalbumin using a fluorescent calcium indicator

✍ Scribed by Marc EBERHARD; Paul ERNE

Book ID
115128992
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
691 KB
Volume
202
Category
Article
ISSN
1432-1327

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Fine tuning the N-terminus of a calcium
Fine tuning the N-terminus of a calcium binding protein: α-lactalbumin
✍ Dmitry B. Veprintsev; Mahesh Narayan; Serge E. Permyakov; Vladimir N. Uversky; C 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 148 KB 👁 1 views

The effects of amino acid substitutions in the N-terminus of bovine recombinant ␣-lactalbumin (including enzymatic removal of the N-terminal methionine and deletion of Glu-1) were studied by intrinsic fluorescence, circular dichroism (CD), and differential scanning microcalorimetry (DSC). Wild-type