Analysis of acid invertase and comparison with acid phosphatase in the ericoid mycorrhizal fungusHymenoscyphus ericae(Read) Korf and Kernan

✍ Scribed by C. J. Straker; W. H. Schnippenkoetter; M.-C. Lemoine

• Book ID: 104662140
• Publisher: Springer-Verlag
• Year: 1992
• Tongue: English
• Weight: 699 KB
• Volume: 2
• Category: Article
• ISSN: 0940-6360
• DOI: 10.1007/bf00203251

✦ Synopsis

Fractions of acid invertase and acid phosphatase of the ericoid mycorrhizal fungus Hymenoscyphus ericae (Read) Korf & Kernan were compared by column chromatography and polyacrylamide gel electrophoresis. Acid invertase levels were measured during the exponential phase after 14 days growth in pure culture. Most acid invertase was wall associated (50%) with 41% forming an extracellular fraction and 9% a soluble, cytoplasmic fraction. The wall-bound fraction was partially solubilized by 1 M NaC1, bulked with the extraceUular fraction and separated by gel filtration into two acid invertase activity peaks. These peaks corresponded closely to two acid phosphatase activity peaks measured in the same eluates. Anion exchange chromatography under a continuous salt gradient separated the invertase and phosphatase isoforms from each other. Non-denaturing polyacrylamide gel electrophoresis demonstrated that the more active isoforms of each enzyme have different electrophoretic properties and are high mannose-type glycoproteins with a high affinity for the lectin, concanavalin A. The results are discussed in terms of the functional aspects of the two enzymes and their cytochemical localization.