Analysis of 6R- and 6S-tetrahydrobiopterin and other pterins by reversed-phase ion-pair liquid chromatography with fluorimetric detection by post-column sodium nitrite oxidation

Determination of d-myo-1,2,6-inositol tr

Determination of d-myo-1,2,6-inositol trisphosphate by ion-pair reversed-phase liquid chromatography with post-column ligand exchange and fluorescence detection

✍ H. Irth; M. Lamoree; G.J. de Jong; U.A.Th. Brinkman; R.W. Frei; R.A. Kornfeldt; 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 615 KB

A detection system for D-myo-1,2,6-inositol trisphosphate (1 ,2,6-IP3) has been developed which is based on the strong complexing properties of the phosphate groups and which includes a post-column ligand-exchange reaction. The weakly fluorescent complex of Fe"' with methylcalcein blue (MCB) is used