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Analysis of 6 VNTR loci by ‘multiplex’ PCR and automated fluorescent detection

✍ Scribed by Gillian Tully; Kevin M. Sullivan; Peter Gill

Publisher
Springer
Year
1993
Tongue
English
Weight
661 KB
Volume
92
Category
Article
ISSN
0340-6717

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✦ Synopsis

The polymerase chain reaction was used to amplify six small variable number of tandem repeat loci in two reactions (D19S20 co-amplifying with D17S5 and D1S80; D17S766 co-amplifying with D16S83 and D17S24). When coupled with fluorescent detection of the products, this provides a rapid, highly discriminating automated test. Preferential amplification of small alleles, leading to 'allelic dropout' was found to occur in D19S20 and D16S83. Population databases are presented for Caucasians and Afro-Caribbeans at loci D19S20, D16S83 and D17S24, and for Asians at D19S20.

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