Analysis of 5-OH-indoles in human gut biopsy tissues by reversed-phase high-performance liquid chromatography with fluorimetric detection

✍ Scribed by B. Viell; K.-H. Vestweber; B. Krause

• Book ID: 103911034
• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 432 KB
• Volume: 6
• Category: Article
• ISSN: 0731-7085
• DOI: 10.1016/0731-7085(88)80113-4

✦ Synopsis

A method was devised for the rapid simultaneous determination of major indoles in human gut tissues. Analysis with picomol detection limits was done by HPLC on a Crs reversed-phase column with fluorimetric detection at 276/350 nm. This simple method for which there is no necessity of derivatization or purification was validated for routine analysis of small mucosa samples (less than 4 mg fresh weight) obtainable during endoscopy. A comprehensive list of 5-OH-indole compounds in human gut tissue is presented.