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Analysis of 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedure

✍ Scribed by G. Volckaert; W.Min Jou; W. Fiers

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 914 KB
Volume: 72
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90551-0

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✦ Synopsis

A modified procedure is described for the analysis of enzymatic digests of 32P-labeled RNA on thin layer plates. The first dimension consists of conventional electrophoresis at pH 3.5 on cellulose acetate and the second dimension is according to chain length by chromatography with an appropriate homomixture. Ribonuclease T,-digests are run on polyethyleneimine thin layer plates and pancreatic ribonuclease digests on diethylaminoethyl-plates.

The procedure combines high sensitivity (due to the small pin-size spots) with excellent resolution. Moreover, the composition of nearly all spots can be deduced from their position. As an illustration, the analyses of enzymatic digests of MS2 RNA (chain length 3569) are presented.

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