Analysis by high-performance liquid chromatography of radioactively labeled carbohydrate components of proteoglycans

✍ Scribed by L.Stefan Lohmander

• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 816 KB
• Volume: 154
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(86)90498-7

✦ Synopsis

Methods were developed for the separation of radioactively labeled carbohydrate components of proteoglycans by isocratic ion-moderated partition HPLC. Neutral sugars were separated after hydrolysis in trifluoroacetic acid with baseline separation between glucose, xylose, galactose, fucose, and mannose. N-Acetylneuraminic acid, N-acetylated hexosamines, glucose, galactose, and xylitol were likewise well separated from each other under isocratic elution conditions. Glucuronic acid, iduronic acid, and their lactones were separated after hydrolysis in formic acid and sulfuric acid. Glucosamine. galactosamine, galactosaminitol. and glucosaminitol were separated by HPLC on a cation exchanger with neutral buffer after hydrolysis in hydrochloric acid. The separation techniques also proved useful in fractionation of exoglycosidase digests of 0-and N-linked oligosaccharicles. Separations of aldoses. hexosamines, and uranic acids were adapted to sensitive photometric detection. 0 1986 Academic Press, 1~.