𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Analysis and simulation of complex interactions during dynamic microfiltration of Escherichia coli suspensions

✍ Scribed by F. Meyer; I. Gehmlich; R. Guthke; A. Górak; W. A. Knorre

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
497 KB
Volume
59
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.

✦ Synopsis

Microfiltration is an important unit operation in downstream processing. However, due to the influence of membrane fouling, prediction of the filtration performance for biological suspensions is difficult. This paper describes a modeling approach that allows a comprehensive description of filtration performance. On the basis of experimental data and linguistic information, a specific artificial neural network was developed that predicts the process behavior within a certain range of parameters. This approach allows us to analyze influences of fermentation on filtration. By using extensive simulations, the interactions of 17 parameters were examined and the fouling causes determined. The model was developed for cell harvesting of Escherichia coli through a shear-enhanced module. The method can be applied to any cross-flow filtration process.

📜 SIMILAR VOLUMES

Analysis of NADPH supply during xylitol
Analysis of NADPH supply during xylitol production by engineered Escherichia coli
✍ Jonathan W. Chin; Reza Khankal; Caroline A. Monroe; Costas D. Maranas; Patrick C 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 259 KB 👁 1 views

## Abstract __Escherichia coli__ strain PC09 (Δ__xylB__, cAMP‐independent CRP (__crp__\*) mutant) expressing an NADPH‐dependent xylose reductase from __Candida boidinii__ (CbXR) was previously reported to produce xylitol from xylose while metabolizing glucose [Cirino et al. (2006) Biotechnol Bioeng

Analysis and use of endogenous nuclease
Analysis and use of endogenous nuclease activities in Escherichia coli lysates during the primary isolation of plasmids for gene therapy
✍ G. A. Monteiro; G. N. M. Ferreira; J. M. S. Cabral; D. M. F. Prazeres 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 261 KB 👁 1 views

Two important issues in the downstream processing of plasmids for gene therapy are the stability of plasmids in the process streams, and the presence of contaminating host RNA. Results with a 4.8-kb plasmid harbored in a non-nuclease-deficient strain of Escherichia coli show that, in spite of the ha

Flow cytometric analysis of bacterial ph
Flow cytometric analysis of bacterial physiology during induction of foreign protein synthesis in recombinant Escherichia coli cells
✍ N. Borth; R. Mitterbauer; D. Mattanovich; W. Kramer; K. Bayer; H. Katinger 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 233 KB 👁 2 views

The production of foreign proteins at high yields represents a severe metabolic stress for Escherichia coli cells. In many cases, induction of protein synthesis results in rapid exhaustion of the cellular energy and metabolic precursors and thus in cell death. Therefore, sustained production of fore

Exploring the dynamic information conten
Exploring the dynamic information content of a protein NMR structure: Comparison of a molecular dynamics simulation with the NMR and X-ray structures of Escherichia coli ribonuclease HI
✍ Marios Philippopoulos; Carmay Lim 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 354 KB 👁 2 views

The multiconformer nature of solution nuclear magnetic resonance (NMR) structures of proteins results from the effects of intramolecular dynamics, spin diffusion and an uneven distribution of structural restraints throughout the molecule. A delineation of the former from the latter two contributions