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Analysis and metabolic engineering of lipid-linked oligosaccharides in glycosylation-deficient CHO cells

โœ Scribed by Meredith B. Jones; Noboru Tomiya; Michael J. Betenbaugh; Sharon S. Krag

Book ID
116300095
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
522 KB
Volume
395
Category
Article
ISSN
0006-291X

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โœ Hudson H. Freeze; Petra Koza-Taylor; Jeffrey A. Jones; William F. Loomis ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 1010 KB

N-glycosylation was measured in wild-type cell lysates of Dictyostelium discoideum and in two mutant strains that synthesize a truncated lipid-linked oligosaccharide, Man,GlcNAc, lacking terminal mannose and glucose residues. Endogenous lipidlinked oligosaccharide (LLO) was transferred to octanoyl-A