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Analysis and characterization of the IncFV plasmid pED208 transfer region

✍ Scribed by Jun Lu; Jan Manchak; William Klimke; Colin Davidson; Neville Firth; Ronald A Skurray; Laura S Frost


Book ID
114121987
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
356 KB
Volume
48
Category
Article
ISSN
0147-619X

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The origin of transfer (oriT) of the con
✍ Coupland, George M. ;Brown, Anthony M. C. ;Willetts, Neil S. πŸ“‚ Article πŸ“… 1987 πŸ› Springer 🌐 English βš– 900 KB

The origin of transfer (oriT) is the sequence within which conjugal transfer of plasmid DNA is initiated, and is absolutely required in cis for plasmid mobilization. We have cloned oriT from the 52 kb IncN plasmid R46 on a 600 bp fragment, and mapped the limits of the relevant sequence by deletion a