Analyse des corps proteiques isolés deLablab purpureus(L.) Sweet: Localisation intracellulaire des globulines, protéases et inhibiteurs de la trypsine
✍ Scribed by Marie-Noëlle Miège; Jean-Michel Mascherpa; A. Royer-Spierer; A. Grange; J. Miège
- Book ID
- 104750706
- Publisher
- Springer-Verlag
- Year
- 1976
- Tongue
- English
- Weight
- 504 KB
- Volume
- 131
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
Analysis of Protein Bodies Isolated from
Lablab purpureus (L.) Sweet: Intracellular Localisation of Globulins, Proteases and Trypsin Inhibitors Summary. Cotyledonary cells are submitted to fractionation by isopycnic centrifugation. Small intact protein bodies are collected in the densest zones (d= 1.205-1.237 g/cm3). Fragments of larger bodies are gathered in zones of lower density (d = 1.205 g/cm3). Small dense bodies are largely sedimentable after dilution, whereas fragments of the large bodies dissociate into a dense sedimentable clot and into floating elements which contain most of the globulins and all of the albumins. Among the dissociated floating components are the BAPA-active endopeptidases and the trypsin inhibitors (BAPA=c~-N-benzoyl-DL-arginine-p-nitroanilide). A caseolytic activity remains with the dense mass. The localisation of the albumins, globulins, proteases and trypsin inhibitors is discussed. Relations between solubility, structure and function are considered.